EFEK PEMBERIAN FRAKSI DIKLOROMETANA BULBUS BAWANG DAYAK (Eleutherine americana) PADA KUALITAS SPERMATOZOA TIKUS PUTIH (Rattus norvegicus) YANG DIPAPAR ASAP ROKOK

Abstract

Cigarette smoke is a source of free radical which possesses a negative impact on reproductive health, especially on sperm quality which affects spermatogenesis process. The aims of this research were to examine the influence of dichloromethane fraction of bawang dayak bulbs on the quality of sperm due to the smoke exposure, to examine the effective dose of dichloromethane fraction of bawang dayak bulbs in preventing the decrease of sperm quality after smoke exposure, and to evaluate difference of antioxidant
ability from dichloromethane fraction and ethanol extract of bawang dayak bulbs with a dose of 90 mg/Kg bw after smoke exposure. This study using the completely randomized design with 24 rats as sample were divided into six treatment groups of 4 mice each. Smoke exposure was conducted for 30 days. The data consisted of sperm morphology, amount, and sperm viability. The quantitative data was analyzed with ANOVA followed by BNT, whereas the qualitative data was showed in picture. The result showed that the smoke-induced rats treated with dichloromethane fraction of bawang dayak bulbs were able to maintain the normal percentage of sperm morphology, amount, and sperm viability. Dose of 90 mg/Kg bw of dichloromethane fraction of bawang dayak bulbs is determined as an effective dose to prevent the decrease of sperm quality after smoke exposure. Therefore, it can be concluded that the antioxidant ability of dichloromethane fraction of bawang dayak bulbs is equal to ethanol extract of bawang dayak bulbs with a dose of 90 mg/Kg bw after smoke exposure.